Multi-Microbial Time Challenge Studies

Anti-Microbial activities of Di-Dak-Sol™

(Does Di-Dak-Sol^TM destroy MRSA?)

Purpose of Study

The purpose of this study was to determine how quickly Di-Dak-Sol™ would kill MRSA, VRE, and 6 other microorganisms commonly found in wounds. To avoid bias, we hired two separate outside laboratories to perform tests. (Di-Dak-Sol™ is a diluted form of Dakin's Solution. Therefore, if Di-Dak-Sol™ kills MRSA, and the other microorganisms, we can assume that higher concentrations of Dakin's Solution will also kill the same microorganisms.)

Double Blind Randomized Testing: Two independent, third party laboratories performed the Mutli-Microbial Time Challenge Studies, according to Century's protocol.

Method

For purposes of the tests, two different batches of Di-Dak-Sol™ (Sodium Hypochlorite 0.0125%) were randomly selected. One of the batches being tested had almost expired. This batch was chosen to confirm that Di-Dak-Sol™ would kill MRSA, even if the Di-Dak-Sol™ was close to expiring.

Both samples, "A" and "B", were sent to two different laboratories for Multi-Microbial Time Challenge Studies.

The first laboratory exposed several types of bacteria to Di-Dak-Sol™ for 15, 30 and 60 minutes. These times were selected because usually Di-Dak-Sol™ is used as the wetting agent for a wet-to-moist dressing, and is kept on the wound for more than 30 minutes.

The second laboratory, in addition to the tests above, exposed MRSA and VRE to Di-Dak-Sol™ for 30 seconds. This was to show that these two resistant bacteria, commonly found in wounds, were killed when they were exposed to Di-Dak-Sol™.

Results

Both MRSA and VRE were killed in a short period of time, even when extremely high concentrations of these resistant bacteria were present.

None of the microorganisms were found at the end of the test, Di-Dak-Sol had eliminated them 100%.

The log reduction column shows the difference between the beginning concentration of microorganisms and the ending concentration. A Log Reduction of ">8" shows that 10 times the bacteria was killed, as compared to a Log Reduction of ">7".

MRSA – Methicillin Resistant Staphylococcus aureus
VRE – Vancomycin Resistant Enterococcus faecalis

Sample "A" 30 seconds	Microorganism Tested	Beginning Concentration	Ending Concentration	Log Reduction
	MRSA ATCC BAA-38	1.94 * 10^ 8	<1 * 10^ 0	>8
	VRE ATCC 700221	1.9 * 10^ 7	<1 * 10^ 0	>7

Sample "A" 15 minutes	Pseudomonas aeruginosa ATCC 9027	1.5 * 10^ 8	<1 * 10^ 0	>8
	Proteus mirabilis ATCC 14153	8.1 * 10^ 7	<1 * 10^ 0	>8
	Escherichia coli ATCC 11775	7.7 * 10^ 7	<1 * 10^ 0	>8
	Serratia marcescens ATCC 13477	1.01 * 10^ 8	<1 * 10^ 0	>8
	MRSA ATCC BAA-38	3.2 * 10^ 7	<1 * 10^ 0	>7
	VRE ATCC 700221	1.8 * 10^ 7 .	<1 * 10^ 0	>7
	Aspergillus niger ATCC16404	8.2 * 10^ 6	7 * 10^ 0	>6
	Candida albicans ATCC10231	7.9 * 10^ 5	<1 * 10^ 0	>6

Sample "A" 30 minutes	Pseudomonas aeruginosa ATCC 9027	4.8 * 10^ 7	<1 * 10^ 0	>7
	Proteus mirabilis ATCC 14153	3 * 10^ 8	<1 * 10^ 0	>8
	Escherichia coli ATCC 11775	3.88 * 10^ 8	<1 * 10^ 0	>8
	Serratia marcescens ATCC 13477	4.27 * 10^ 8	<1 * 10^ 0	>8
	MRSA ATCC BAA-38	1.94 * 10^ 8	<1 * 10^ 0	>8
	VRE ATCC 700221	1.9 * 10^ 7 .	<1 * 10^ 0	>7
	Aspergillus niger ATCC16404	4.3 * 10^ 7	7 * 10^ 0	>6
	Candida albicans ATCC10231	4.7 * 10^ 6	<1 * 10^ 0	>6

Sample "A" 60 minutes	Pseudomonas aeruginosa ATCC 9027	4.8 * 10^ 7	<1 * 10^ 0	>7
	Proteus mirabilis ATCC 14153	3 * 10^ 8	<1 * 10^ 0	>8
	Escherichia coli ATCC 11775	3.88 * 10^ 8	<1 * 10^ 0	>8
	Serratia marcescens ATCC 13477	4.27 * 10^ 8	<1 * 10^ 0	>8
	MRSA ATCC BAA-38	1.94 * 10^ 8	<1 * 10^ 0	>8
	VRE ATCC 700221	1.9 * 10^ 7 .	<1 * 10^ 0	>7
	Aspergillus niger ATCC16404	4.3 * 10^ 7	<1 * 10^ 0	>7
	Candida albicans ATCC10231	4.7 * 10^ 6	<1 * 10^ 0	>6

Sample "B" 15 minutes	Pseudomonas aeruginosa ATCC 9027	1.5 * 10^ 8	<1 * 10^ 0	>8
	Proteus mirabilis ATCC 14153	8.1 * 10^ 7	<1 * 10^ 0	>8
	Escherichia coli ATCC 11775	7.7 * 10^ 7	<1 * 10^ 0	>8
	Serratia marcescens ATCC 13477	1.01 * 10^ 8	<1 * 10^ 0	>8
	MRSA ATCC BAA-38	3.2 * 10^ 7	<1 * 10^ 0	>7
	VRE ATCC 700221	1.8 * 10^ 7 .	<1 * 10^ 0	>7
	Aspergillus niger ATCC16404	8.2 * 10^ 6	7 * 10^ 0	>6
	Candida albicans ATCC10231	7.9 * 10^ 5	<1 * 10^ 0	>6

Sample "B" 30 minutes	Pseudomonas aeruginosa ATCC 9027	4.8 * 10^ 7	<1 * 10^ 0	>7
	Proteus mirabilis ATCC 14153	3 * 10^ 8	<1 * 10^ 0	>8
	Escherichia coli ATCC 11775	3.88 * 10^ 8	<1 * 10^ 0	>8
	Serratia marcescens ATCC 13477	4.27 * 10^ 8	<1 * 10^ 0	>8
	MRSA ATCC BAA-38	1.94 * 10^ 8	<1 * 10^ 0	>8
	VRE ATCC 700221	1.9 * 10^ 7 .	<1 * 10^ 0	>7
	Aspergillus niger ATCC16404	4.3 * 10^ 7	3 * 10^ 0	>7
	Candida albicans ATCC10231	4.7 * 10^ 6	<1 * 10^ 0	>6

Sample "B" 60 minutes	Pseudomonas aeruginosa ATCC 9027	4.8 * 10^ 7	<1 * 10^ 0	>7
	Proteus mirabilis ATCC 14153	3 * 10^ 8	<1 * 10^ 0	>8
	Escherichia coli ATCC 11775	3.88 * 10^ 8	<1 * 10^ 0	>8
	Serratia marcescens ATCC 13477	4.27 * 10^ 8	<1 * 10^ 0	>8
	MRSA ATCC BAA-38	1.94 * 10^ 8	<1 * 10^ 0	>8
	VRE ATCC 700221	1.9 * 10^ 7 .	<1 * 10^ 0	>7
	Aspergillus niger ATCC16404	4.3 * 10^ 7	3 * 10^ 0	>7
	Candida albicans ATCC10231	4.7 * 10^ 6	<1 * 10^ 0	>6